Part:BBa_K2992049:Design
FAST reporter construct with PLac
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 573
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 573
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 573
Illegal BglII site found at 633 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 573
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 573
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1600
Design Notes
Amplified unmodified from the genome sequences of the host organisms. The bgaRL regulatory unit has been experimentally tested in terms of its application but not its function. The promoter prediction tool BPROM (Zuker, 2003) was used to identify putative -35 and -10 boxes from which the promoter regions and UTRs were predicted for the series of entries relating to the BgaRL system. Owing to the bidirectional nature of the bgaRL system, all bgaRL entries are listed 5'-3'-exactly as found on the chromosome of C. perfringens
Source
C. pasteurianum and C. sporogenes DNA. FAST is originally derived from Halorhodospira halophile but codon optimised for Clostridium spp. [Street et al, 2019 DOI: 10.1128/AEM.00622-19].